Electrochemical Aptasensors with Nonlabeled Redox Probes

4 Electrochemical Aptasensors with Nonlabeled Redox Probes 

An electrochemical sensor with a nonlabeled redox probe shakes off probes labeled on the apatmers but still requires that the probes indicate changes on the sensing interface. One commonly nsed probe, methylene bine (MB), belongs to the phenothiazine family and is an aromatic cationic dye showing optically and electrochemically active properties (Tuite and Norden, 1994). Usually, MB can bind with dsDNA or tRNA via intercalation, electrostatic absorption, or G-base binding, which has been nsed widely in DNA sensors and recently, in aptasensors (Tuite and Norden, 1994 Bang et ah, 2005). 

---