ECEEM-Based Selection of Aptamers

In an experiment, peak broadening and nonspecific interactions with other components in the system may introduce a bias of unbound ligands into the fraction collected. That is why the selection procedure may require a few rounds of SELEX to approach the theoretically predicted Xd values. 

It is necessary to know and 1l t for ECEEM-based selection of smart aptamers. These two parameters can be obtained from a single NECEEM 

The unique featme of ECEEM for the selection of aptamers arises from its simple mathematical description. The range of the affinity distribution along the capillary is determined by [T], Tl t and Tl- Changing these parameters can theoretically facifitate the selection of aptamers with affinities ranging from picomolar to millimolar values of Ki. It should be noted that the simple mathematical description is applicable only under an assumption of quasiequilibrium in the system. The reequilibration time in reaction (9.1) should be much shorter than the characteristic time of electrophoretic separation. 

Example So far, experimental evaluation of ECEEM has been conducted only for the selection of DNA aptamers for MutS protein (Drabovich et al., 2005, 

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