Dynamic range desorption/ionization

For analytes with unknown ionization/desorption properties, typically pilot runs at various sample amounts are recommended that determine the dynamic range of MALDI-TOF detection from TLC plates. Typical application volumes are between 2 and 10 pL/band. Drying of the start zones follows, for example, on a TLC plate heater set at 60°C for 1 min. Chromatography, documentation, and quantification can be performed as mentioned before (Section 49.3.1.3). 

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