Duplex linear oligomers

Fig. 4. A model of the nucleotide sequence arrangement contained within AAV DNA, Two nucleotide sequence permutations are illustrated. Plus and minus strands may anneal to form duplex linear monomers with (3 and 4) or without cohesive 3 or 5 termini (1 and 2). Duplex linear monomers with cohesive termini can then form duplex circular monomers or duplex linear oligomers. In the figure the terminal repetitions are depicted as symmetrical nucleotide sequences. In the inset two alternative types of terminal repetitions are illustrated the first has the inverted repetition subterminal to the natural repetition, the second illustrates the possibility that a strand may have either an inverted or a natural terminal repetition...

Fig. 5- Duplex AAV linear monomers which did not form circles or oligomers when exposed to annealing conditions (< 1 %) were digested to the extent of 1 % using exonuclease III and annealed in 2 X SSC (0.30 M NaCl, 0.03 M Na citrate) at either 45° C ( ) or 55° C (o) for various periods of time. The percentage of circles and dimers formed is plotted. (No dimers were observed at 55° C)...

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