Dulbecco’s minimal essential medium

Fig. 2.2 Stability of IgCi monoclonal antibody added to sterile plant and animal cell culture media. ( ) Murashige and Skoog (MS) medium (A) Dulbecco s minimal essential medium (DMEM) with 10% serum and (A) serum-free Ex-cell 302 medium. The error bars indicate standard errors from triplicate flasks. (Reproduced with permission, from B. M. -Y. Tsoi and P. M. Doran, Biotechnol. Appi. Bio-chem. 2002, 35, 171-180. Portland Press on behalf of the IUBMB.)...

Dulbecco s Minimal Essential Medium (Dulbecco s Modified Eagle s Medium, MEM Dulbecco, DMEM)... 

Fig. 1. The 500-MHz Hahn spin-echo H NMR spectra of a cell culture medium, Dulbecco s minimal essential medium, before (A) and after (B) reaction with 400 iiM cisplatin for 24 hr at 37°C, and after (C) reaction with Pt(en)Cl2l. Note the disappearance of the singlet at 2,14 ppm for the S-methyl of L-Met, which has formed Pt-Met complexes, giving new peaks at —2.7 ppm. (Adapted from Ref. 23.)...

A sterile, well-buffered medium, such as Dulbecco s minimal essential medium (DMEM) without phenol red or sodium bicarbonate, supplemented with 20 mM HEPES and set at pH = 7.4 at 37°C, (which is 7.58 at room temperature because of temperature coefficient of HEPES). Store sterile at 4°C. 

Dulbecco s minimal essential medium (DMEM) containing 1% penicillin/streptomycin. 

Culture medium Dulbecco s minimal essential medium (DMEM) supplemented with 10 % fetal bovine serum (FBS), 1 % glutamine, and 100 Upenicillin/streptomycin (e.g., Celbio, Pero, Italy)... 

Tissue-culture media endotoxin-free Dulbecco s modified Eagles medium (DMEM), minimal essential medium-alpha (MEMa) or Hepes-buffered RPMI-1640 supplemented with 2 mM r-glutamine, and heat-inactivated fetal bovine serum (FBS, to 10% final concentration) (see Note 1). Store at 4 °C. 

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