DsRED chromophore formation

Fig. 5.2. Chromophore formation in avGFP and DsRed. Chromophore formation in avGFP (A) requires folding of the tripeptide into the right conformation in order to enable cyclization and oxidation to form the mature green chromophore. In DsRed (B) chromophore formation follows the same path as for avGFP but requires an additional oxidation step to extend the conjugation of the chromophore.

Fig. 2 Sdiematk of folding, chromophore formation, and chromophore modification of DsRed. The p-can structure represents the native conformation of the protein, while the denatured form is shown as an irregular chain. It is not known at which step oligomerization occurs, n-con-jugation for visible-light absorption is indicated in gray. Reprinted with permission from Miyawaki et al., Curr. Opin. Chem. Biol. 7,560 (2003). Copyright 2003 Elsevier...

Fig. 14.30 Proposed mechanism of formation of the red fluorescent chromophore from amino acid side chains in DsRed.

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