Drug development dilution linearity

To run a patient sample, you will need to go through exactly the same deproteination, SFE cartridge extraction, IS addition, mobile phases dilution, and injection steps (Fig. 12.4f). From the peak heights relative to the IS height, we can now quantitate the amount of each drug in the patient s blood. To insure linearity, you may need to dilute our windowed plasma blank and spike it with different levels of each standard and plot calibration curves for each compound, but basically, our methods development is done. 

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