Down-fill, slurry column packing

Slurry packing techniques are required for the preparation of efficient columns with rigid particles of less than 20 micrometers in diameter. The same general packing apparatus. Figure 4.8, can be used to pack columns by the balanced-density slurry, liquid slurry, or the viscous slurry techniques. Down-fill slurry packing is the method of choice for small bore columns and packed capillary columns. 

Carefully pack a clean column by first filling with a third column volume of buffer (0.2M sodium phosphate, pH 6 0, containing 0.1M sodium sulfate) Swirl the slurry to resuspend it evenly, and pour it down a glass rod onto the inside wall to fill the column Allow to settle under gravity for 0.5-1 h and to let air bubbles escape (see Note 12)... 

Transfer about a quarter of this slurry to the column (Note (g)) filled with water and gently press small quantities of this slurry down with a glass plunger so that an evenly packed column of the KelF/TBP is formed. Note (h). 

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