Fragmentation spectrum doubly-charged precursor

Fig. 1.45 Example of a 2D nano LC-MS/MS analysis of a C elegans extract. (A) Fraction 2, 4 mM KCI salt elution on the strong cation exchange column. (B) Full scan MS spectrum of the peak eluting at RT 26.3 min in (A). (C) product ion spectrum of the doubly charged precursor of (B) at m/z 784.8. Y fragments are typical for C-terminal fragments while b ions are typical for N-terminal fragments.

Figure 6.19. Fragmentation spectrum of doubly charged peptide precursor at 741.0Th, used in Example 3.

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