Double-label immunocytochemistry

Pitt JC, Lindemeier J, Habbes HW, Veh RW (1998) Haptenylation of antibodies during affinity purification a novel and convenient procedure to obtain labeled antibodies for quantification and double labeling. Histochem Cell Biol 110 311 322 Polak JM, Van Noorden S (1997) Introduction to immunocytochemistry. BIOS Scientific... 

Fig. 143. Comparison between Purkinje cells of the A and B zone (open circles) retrogradely labelled from the vestibular nuclei (left side) and retrograde labelling of Purkinje cells of the X zone after an injection of WGA-HRP in the transitional region of the fastigial and posterior interposed nucleus the rat (right side). Purkinje cells of the X zone occupy the Zebrin-positive P2+ zone labelled Purkinje cells of the lateral A zone and the B zone are located in the Zebrin-negative PI- and P2- zones. Graphical reconstructions of transverse sections double labelled for HRP reaction product and Zebrin 1 immunocytochemistry. COP = copula pyra-midis CrI and II = crus I and II of the paramedian lobule PMD = paramedian lobule SI = simple lobule I-X = lobules I-X. Voogd et al. (1991b).

Angaut P, Sotelo C (1989) Synaptology of the cerebello-olivary pathway. Double labelling with anterograde axonal tracing and GABA immunocytochemistry in the rat. Brain Res., 479, 361-365. 

Egger, D, Troxler, M., and Bienz, K (1995) Light- and electron microscopic in situ hybridization- nonradioactive labeling and detection, double hybridization, and combined hybridization—immunocytochemistry J Histochem Cytochem 42, 815-822... 

Mason, D Y., Abdulaziz, Z, Falini, B., and Stem, H (1983) Double immunoenzymatic labeling, in Immunocytochemistry, Practical Applications in Pathology and Biology (Polak, J M and VanNoorden, S., eds.), Wright PSG, Bristol, England, pp. 113-128... 

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