Double jump

Fig. 32. Double-jump experiments of unfolding and refolding. The peptide [(Ala-Gly-Pro)s]3 in 50 ml phosphate buffer (pH 7.5) was incubated at 9.2 °C and quickly unfolded by a first temperature jump from 9.2 to 30 °C. This process took 25 s, the time needed to reach the final temperature. In a first experiment (curve A), the second jump back from 30 to 9.2 °C followed immediately after complete unfolding of the peptide, i.e. 25 s after the first jump. In a second and a third experiment (curve B, C), the time lapse between the first and the second jump was 75 and 125 s, respectively...

That peptidyl-proline isomerization is a post-unfolding event is readily demonstrated by double-jump experiments.17 The protein is denatured for several milliseconds and then restored to renaturing conditions before the peptidyl prolines have had time to isomerize. The proline-related phases are then no longer seen. 

Figure 15 Schematic diagram of a diffusing C-type trough dimer along die trough, (a)-(b) Refers to a single jump along the trough and (a)-(c) to a double jump. Large and small circles represent atoms that buckle upwards and downwards, respectively.

Table 4.13. Single and double jump ring-puckering transitions observed in the Raman effect for trimethylene imine90)...

Double jump techniques reveal CTI by time-dependent amplitudes of folding phases. 

Jacobsen, J., Jacobsen, K.W., Sethna, J.P. Rate theory for correlated processes Double jumps in adatom diffusion, Phys. Rev. Lett. 1997, 79,2843. 

Figure 6.56 Results of a vigilance study vigilance performance (detection of double jumps of an instrument pointer) as a function of the duration of the monitoring activity.

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