Dopamine, standard curves

Figure 15. Standard curves for norepinephrine and dopamine vs. dihydroxybenzylamine as an internal standard (Kawasaki, T. Wong, 0. Imai, K. Higuchi, T., in preparation).

Figure 9.S Standard curves of Dopa and dihydroxybenzylamine by HPLC with voltammetric detection for the peak height. One hundred microliters of a sample containing various amounts (500 fmol to 100 pmol) of dopamine (1) and hydroxybenzyl-amine (2) was injected into the column and was detected by a voltammetric detector. (From Nagatsu et al., 1979.)...

Fig. 4.4 SERS method for dopamine (DA) detection using Au Ag NR dimers building by aptamer. a Detection scheme, b The standard curve for the DA detection, where the SERS intensities were calculated at the wavenumber 1075 cm of Raman reporter (adapted with permission from Tang et al. 2015a. Copyright 2015 Elsevier B.V.)...

It is appropriate at this juncture to illustrate the power of chemiluminescence in an analytical assay by comparing the limits of sensitivity of the fluorescence-based and the chemllumlnescence-based detection for analytes in a biological matrix. The quantitation of norepinephrine and dopamine in urine samples will serve as an illustrative example. Dopamine, norepinephrine, and 3,4-dihydroxybenzy-lamine (an internal standard) were derivatized with NDA/CN, and chemiluminescence was used to monitor the chromatography and determine a calibration curve (Figure 15). The limits of detection were determined to be less than 1 fmol injected. A typical chromatogram is shown in Figure 16. 

---

See also in sourсe #XX -- [ , ]

---