Documentation autoradiography

Autoradiography. Autoradiography is one of the oldest methods used for semiquanti-tative measurement of radioactivity. The chromatogram is placed on an x-ray film and exposed in the dark for usually less than 1 h (a detailed procedure is given elsewhere) (Theobald 1994). This method is no longer in use since it is time-consuming and inaccurate. Visualization of the radioactivity distribution on a film may be useful for documentation purposes. 

Autoradiography can be used to document chromatograms containing samples labeled with radioactive isotopes (see Chapter 13). Digital autoradiography combined with TLC-MS-MS was used for the structure determination of drug metabolites (Ludanyi, 1997). 

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