DNases endonucleases

Sikora E, Bielak-Zmijewska A, Magalska A, Piwocka K, Mosieniak G, Kalinowska M, Widlak P, Cymerman IA, Bujnicki JM. 2006. Curcumin induces caspase-3-dependent apoptotic pathway but inhibits DNA fragmentation factor 40/caspase-activated DNase endonuclease in human Jurkat cells. Mol Cancer Ther 5 927-934. 

With lightly alkylated DNA, endonuclease II makes predominantly single-stranded breaks suggesting that the enzyme can hydrolyze a phos-phodiester bond at or near an alkylated base in a native DNA molecule with no single-stranded breaks in this region. With more extensively alkylated DNA, double-stranded breaks predominate. 

M. Cerven, A. Domingo, and R. Mrico. Properties of the dot assay, an easy quantitative assay of DNA endonucleases. Anal. Biochem, J69 41-48 (1988). 

Micrococcal nuclease Staphylococcus aureus RNA and DNA endonuclease splits d bonds in areas rich in adenosine, uracil, and thymine... 

You have a DNA endonuclease that cleaves the DNA internally to generate 3 -OH and 5 -phosphate groups, intact DNA polymerase I, and radioactive dNTPs. Suggest a means for making the DNA radioactive. 

Certain DNA endonucleases degrade double-strand DNA to yield mononucleotides and dinucleotides, but these enzymes do not degrade those duplex sequences to which other proteins are tightly bound. 

RNA polymerase the RNA polymerase will bind tightly to the promoter site. Next, you would add the DNA endonuclease, which will degrade the DNA that is not protected by the bound RNA polymerase. Electrophoresis can then be used to determine the size of the protected fragments of DNA, and the base sequence can be determined using methods discussed in Chapter 6 of the text. 

Engel ML, Ray DS. A structure-specific DNA endonuclease is enriched in kinetoplasts purified from Crithidia fasciculata. Nucleic Acids Res 1998 26(20) 4733-4738. 

Mazumder, A., Gerlt, J. A., Absalon, M. J., Stubbe, J., Cunningham, R. P., Withka, J., and Bolton, P. H. (1991). Stereochemical studies of the beta-elimination reactions at aldehydic abasic sites in DNA Endonuclease III from Escherichia coli, sodium hydroxide, and Lys-Trp-Lys. Biochemistry 30, 1119-1126. 

I., Hauer, M. et al. (2012) A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science, 337, 816-821. 

The experiment shown in Fig. 4 also suggests that DNA endonucleases were not among the poly(ADPR) proteins since neither degradation nor nicking of the circular DNA occurred. The possible presence of topoisomerase II as argued from a 170 kD... 

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