DNA restriction digest

Pure HDF injection can be achieved for sample introduction by using vacuum suction. The sample was sucked through an inserted capillary into a short section of microchannels between three ports. Different amounts could be selected by filling different sections of the microchip [813]. Pressure injection of a DNA sample was also achieved via a transfer capillary sequentially to a five-channel microchip. The use of one capillary allowed for automated sampling necessary for continuous monitoring of an enzymatic DNA restriction digestion experiment [320],... 

Figure 2. Southern analysis of mildew DNA restriction digests probed with plasmid pUC8 containing the sterol 14 a demethylase sequence from yeast, (pDC8-2860H), the whole coding sequence (A), 3 end (B) and 5 end (C).

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