DNA nanotubes

Douglas SM, Chou JJ, Shih WM. DNA-nanotube-induced align- 58. ment of membrane proteins for NMR structure determination. 

Hariadi RF, Yurke B (2010) Elongational-flow-induced scission of DNA nanotubes in laminar flow. Phys Rev E 82 046307... 

Liu, D., Park, S.H., Reif, J.H., Labean, T.H., 2004. DNA nanotubes self-assembled from triple-crossover tiles as templates for conductive nanowires. Ptoc. Natl. Acad. Sd. USA 101, 717-722. 

Figure 14.16. Scheme for fabrication of covalently linked DNA-nanotube... 

Figure 5 DNA nanotubes by self-assembly of a single sequence, (a) Sequence of the four DNA segments, (b) two copies of the strand bind to create two double helices that crossover in two positions, leaving four single-stranded ends. These single-stranded ends can bind to others to form a lattice (c) that binds to form the nanotube (d).

Recently, the preparation of liquid crystal of DNA nanotubes (0.8 J,m in length) that can be used to induce weak alignment of membrane proteins has been described." The nanotubes are heterodimers of 6HBs (each 0.4 pm in length), each self-assembled from a 7.3-kb scaffold strand and >170 short oligonncleotide strands. 

Figure 16 Models of DNA nanotube formation thiough three different strategies based on the rolling mechanism of DNA sheets, (a) DX tile (Reproduced from Ref. 83. American Chemical Society, 2004), (b) two-dimensional, and (c) triple-crossover tile strategies. (Reproduced with permission from Ref. 84, D. Liu, S. H. Park, J. H. Reif, and T. H. LaBean, Proc. Natl. Acad. Sci. USA, 2004, 101, 717. Copyright (2004) National Academy of Sciences, U.S.A.)...

Figure 25 DNA nanotubes derived from cyclic oligomers that in the presence of a complementary DNA strand form the basic nanotube subunit, in which eight single strands of DNAs (in red) are hanging. The addition of a new complementary DNA strand (pink) gives rise to the tubular structure.

A recent approach to create covalently linked DNA nanotubes was reported by Willner and colleagues (Figure 3i). The group created three building blocks bis-amide and bis-thiolated linkers and a circular DNA modified at its opposite poles with thiol and amine units. First, the bis-amide linker was added to cross-link the circnlar DNA, followed by addition of the bis-thiolated linker to yield nanotubes. 

It is also possible to apply engineering techniques to natural polymers like DNA. Indeed, DNA nanotubes are attractive because the structural, energetic, and dynamic control accessible with so-called DNA tiles is unsurpassed. Potential uses of DNA nanotubes are inspired by nanofilaments and nanotubes in living cells. ... 

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