DNA affinity chromatography

Before the era of artificially recombinant DNA, affinity chromatography emerged as a potentially highly selective approach to protein purification [13]. It revived a laborious art based on selective precipitations and a limited range of chromatographic media. 

Specialized Purification Procedures Purification of membrane proteins, 182, 499 purification of integral membrane proteins, 104, 329 reconstitution of membrane proteins, 104, 340 purification of DNA-binding proteins by site-specific DNA affinity chromatography, 182, 521 purification of glycoproteins, 182, 529 purification of multienzyme complexes, 182, 539. 

Enz)mies, inhibitors, cofactors, nucleic acids, hormones or cell chromatography can also be utilized as ligands in bioaffinity chromatography types. Examples of these methods include Receptor Affinity Chromatography and DNA Affinity Chromatography [21]. 

Since then several transcription factors have been purified using DNA-affinity chromatography. The application of DNA-affinity chromatography is not limited to purification of transcription factors. All DNA binding proteins which show sequence specificity can be purified by this technique. 

Moxley RA, Oak S, Gadgil H, Jarrett HW. DNA affinity chromatography. In Hage DS, editor. Handbook of affinity chromatography. 2nd ed. Boca Raton, FL CRC Press 2005. p. 173-214. 

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