Dissociation Constants for Different Substrates

Values for Km, the dissociation constant of the active, enzyme-substrate complex, for mammalian /3-glucuronidase and various substrates are shown in Table V. Table VI gives the values of Km that have been recorded for non-mammalian /3-glucuronidase preparations. Most of the figures have 

When this treatment was applied to /8-glucosidase (/8-D-glucopyranosidase),m discrepancies were noted between and Km when the latter was determined directly for the same compound. Such discrepancies can be explained in two ways. If the en- 

Dissociation Constants (Km) of the Active Enzyme-substrate Complex for Mammalian -Glucuronidase and Various Substrates 

Aglycon Source and Purity of Enzyme pH of Test Km (M) Refer- ences 

Km is based on measurements of the over-all process, whereas competitive inhibition 

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