Disc electrophoresis — general considerations and solutions

Before use the electrode buffer is diluted 1 10. For the preparation of the separation gel 1.5%) the above solutions are mixed in following proportions  

The anode is joined to the bottom, the cathode to the upper part of the device. 

For some special separations (for the separation of biopolymers with p/ above 

5) acidic gels can be used in this case the following composition of solutions is. recommended. 

Polarity of the system is reversed in comparison with the alkaline system, e.g., the anode is mounted at the top, the cathode to the bottom of the apparatus. 

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