Dipolar couplings, nuclease

Shortle has focused on the unfolded state for more than a decade, leading up to his recent demonstration using residual dipolar couplings that staphylococcal nuclease retains global structure in 8 M urea. His chapter on The Expanded Denatured State sets the stage. Dunker etal. then explore the complementary world of disordered regions within... 

Fig. 4. Scatterplots of the Nh residual dipolar couplings from three different denatured states of staphylococcal nuclease plotted against each other. (A) Wild-type, full-length nuclease in 4 M urea (y-axis) versus A131A in buffer (x-axis). (B) Wild-type, full-length nuclease denatured by acid (25 mM citrate, pH 3.0) (y-axis) versus A131A in buffer (x-axis).

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