Dioxygenases three component

The corresponding dioxygenase from Pseudomonas aeruginosa 142 is, however, a three-component enzyme (Romanov and Hausinger 1994). 

Romanov V, RP Hausinger (1994) Pseudomonas aeruginosa 142 uses a three-component orf/ro-halobenzoate 1,2-dioxygenase for metabolism of 2,4-dichloro- and 2-chlorobenzoate. J Bacteriol 176 3368-3374. 

Nam J-W, H Nojiri, H Noguchi, H Uchimura, T Yoshida, H Habe, H Yamane, T Omori (2002) Purification and characterization of carbazole l,9a-dioxygenase, a three-component dioxygenase system of Pseudomonas resinovorans strain CAIO. Appl Environ Microbiol 68 5882-5890. 

Bfinz PV, AM Cook (1993) Dibenzofuran 4,4a-dioxygenase from Sphingomonas sp. strain RWl angular dioxygenation by a three-component system. J Bacteriol 175 6467-6475. 

Dioxygenases that form ds-diols are composed of two or three components, forming ds-dihydrodiols or ds-diol carboxylic acids, respectively. The three-component dioxygenases are composed of a flavoprotein, a ferredoxin, and a terminal oxygenase. 

Lee, K. F., Parales, R., Parales, J. V., Gibson, D. T., and Ramaswamy, S. 2005. Purification, crystallization and preliminary x-ray diffraction studies of the three components of the toluene 2,3-dioxygenase enzyme system. Acta Crystallographica (F Structural Biology and Crystallization Communications), F61 (7), 669-672. 

Class III Three component type e.g. naphthalene dioxygenase... 

Robinson, K.G., W.S. Farmer, and J.T. Novak. 1990. Availability of sorbed toluene in soils for biodegradation by acclimated bacteria. Water Res. 24 345-350. Romanov, V. and R.P. Hausinger. 1994. Pseudomonas aeruginosa 142 uses a three-component orffo-halobenzoate 1,2-dioxygenase for metabolism of 2,4-dichloro-and 2-chlorobenzoate.. Bacteriol. 176 3368-3374. 

Naphthalene dioxygenase consists of three components, which form an electron transfer chain an NADH-dependent flavoprotein reductase, a ferredoxin containing two [2Fe2S] Rieske iron-sulfur clusters, and a Rieske oxygenase containing both a [2Fe2S] Rieske iron-sulfiir cluster and a mononuclear iron(II) center in the enzyme active site. ° ... 

Benzene dioxygenase is a complex enzyme consisting of three protein components, that catalyse the conversion of benzene to benzene cis-dihydrodiol. Give two reasons why this biotransformation should be carried out using whole cells as opposed to using enzyme preparations. 

Benzene 1,2-dioxygenase was first demonstrated in cell-free extracts of a strain of Pseudomonas putida by Gibson et al.203. They were able to resolve the system into two fractions by (NH4)2S04 precipitation, both of which are necessary for benzene oxidation. Subsequently, Axcell and Geary82 obtained a soluble enzyme system which oxidizes benzene to ns-l,2-dihydrocyclohexa-3,5-diene (m-benzeneglycol) from a species of Pseudomonas grown on benzene as the major carbon source. The system is shown to consist of three protein components. Two of these are nonheme... 

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