Dioxetanes analytical applications

The synthesis of A-methylacridan dioxetane derivatives would be very promising for analytical application as chemiluminescence probes. Despite the high quantum yields... 

Their purification is equally problematic. In view of the thermal ability and ease of catalytic decomposition of the four-membered ring peroxides, many of the usual methods of purification are not applicable. This problem is more severe for the more labile a-peroxylactones than the 1,2-dioxetanes. To the best of our knowledge, no analytically pure a-peroxylactones have been prepared to date. ... 

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