Dinitrophenylhydrazones of carbonyl compounds

This method describes the quantitative determination of carbonyl compounds in fats and oils. It is based upon the formation of 2,4-dinitrophenylhydrazones of carbonyl compounds in the presence of a trichloroacetic acid (TCA) catalyst, followed by colorimetric determination of the hydrazone compounds (i.e., as quinoidal ions) in an alkanolic basic solution. 

Dinitrophenylhydrazones of carbonyl compounds may be formed as described for keto steroids (Section 4.1.1.4.1) by precipitation or extraction from aqueous perchlorate as described by Neuberg et al. [65], by the method of Houben-Weyl [66] or by the method of Shriner et al. [67] using sulfuric acid—water-ethanol as the reaction medium. 

Analysis of 2,4-dinitrophenylhydrazones of carbonyl compounds in ambient air that included aliphatic and aromatic aldehydes and ketones (Kolliker et al. 1998). 

Formation of 2,4-dinitrophenylhydrazones of carbonyl compounds is a popular method for their derivatization. However, owing to low volatility of these derivatives, their reversed-phase high-performance liquid chromatography (HPLC) analysis seems more preferable than GC analysis. ... 

Table 27. Survey of the possibilities for chromatographic separation of dinitrophenylhydrazones of carbonyl compounds (2,4-DNP)... 

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See also in sourсe #XX -- [ ]

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