2.4- Dinitrophenylhydrazone, of acetone

Separation of 2,4-dinitrophenylhydrazones. The solutions are prepared by dissolving 10 mg of each of the 2,4-dinitrophenylhydrazones of acetone, butan-2-one and hexan-3-one (or hexan-2-one) in 0.5 ml of ethyl acetate. Prepare a flexible silica gel sheet of dimensions 20 x 5 cm in the manner already described and apply c. 0.5 pi of each of the three solutions to give the marker spots of a diameter of between 2 and 3 mm. A mixed spot is conveniently obtained by loading sequentially to the same area further 0.5 pi aliquot portions of each of the solutions and allowing the solvent to evaporate completely between each addition. 

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