Dimethyl sulfoxide gradients

Complete N NMR chemical shifts together with H- N coupling constants (referenced externally to NH4C1 in DMSO-1 6 DMSO - dimethyl sulfoxide) have been determined for toxoflavin 7 and fervenulin 8 in CDCI3 using pulsed field gradient heteronuclear multiple bond correlation (HMBC) techniques <2000H(52)811>, and are presented in Table 1. 

Cory and Garroway [13] introduced the NMR pulsed gradient stimulated echo method to study compartments which are too small to be observed by conventional NMR imaging. They showed so-called proton displacement profiles of bulk water and dimethyl sulfoxide. The displacements are due to free diffusion and are Gaussian shaped. The profile of water in yeast cells showed restricted diffusion with a characteristic cell width of approximately 5 /xm. 

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