Dihydroxybenzoic small-molecule MALDI

MALDI matrix is typically a small organic acid that co-crystallizes with analytes extracted from the tissne section to allow absorption of energy of the pulsed laser. Matrix and analytes are ablated and ionized from the tissne snrfece with subsequent MS analysis. Common matrixes used for the analysis of peptides, lipids, and small drng molecules are 2,5-dihydroxybenzoic acid (DHB) and a-cyano-4-hydroxycinnamic acid (CHCA) while sinapinic acid (S A) is most often used for protein analysis. 1.23,26,27) An organic solvent snch as acetonitrile or methanol can be used for extraction of small molecules from tissne sections prior to matrix co-crystallization. ... 

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