Digestive Lipases During Lipolysis Method B

Inhibition of Digestive Lipases During Lipolysis (Method B) 

The hydrolysis of tributyrin by HGL and RGL was recorded at pH 6.0 and at pH 8.0 with PPL. Two minutes after enzyme addition, orlistat was injected at variable final concentrations. As can be seen from the kinetic curves in Fig. 9.17, the rate of tributyrin hydrolysis by PPL (Fig. 9.17A), RGL (Fig. 9.17B) and HGL (Fig. 9.17C) decreased to various extents. This decrease was more rapid at higher orlistat concentrations. With all lipases tested, more than 90% of the enzyme inhibition occurred at a final orlistat concentration of 200 pM [51]. 

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