Digested glycopeptides, selective enrichment

Ding, W., Hill, J.J., and Kelly, J. Selective enrichment of glycopeptides from glycoprotein digests using ion-pairing normal-phase liquid chromatography. Anal. Chem. 2007, 79, 8891-8899. 

A shotgun glycoproteomics approach similar to that discussed in Section 9.12.2.4.2 has also been described,119 various steps of which are cleavage of glycoproteins, enrichment of glycopeptides in the digest by selective capture onto a modified solid support, release of peptides by PNGase treatment, and MS/MS analysis of the released peptide. Finally, the database search identifies the proteins. 

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