Diffusion real-time visualization

To test the hypothesis that Sup35-GFP coalescence was providing real-time visualization of the conversion process, the fusion protein was expressed in [psi cells for a longer period. Fluorescence begins to coalesce into discrete foci several hours after diffuse fluorescence is first detected. When these cultures are plated to media that selects for [PS7+] cells but not for the plasmid, [PS/+] cells can be isolated concomitant with the appearance of foci. Thus, the de novo appearance of these complexes of Sup35 is linked to the de novo acquisition of the [PS7+] state (Patino et al., 1996 Zhou etal, 2001). 

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