Diethyl ethoxymethylenemalonate DEEMM

Diethyl ethoxymethylenemalonate (DEEMM) is usually used for amino acid analysis (Alaiz et al., 1992). Recently, a HPLC-photodiode array method using DEEMM as labelling reagent for analysis of BAs in wine, has been developed (see Figure 4.17). 

Table 4.5 HPLC eluent program used for determination of biogenic amine-diethyl ethoxymethylenemalonate (DEEMM) derivatives. Eluent A) 25 mM acetate buffer (pH 5.8) with 0.02% sodium azide eluent B) 80% acetonitrile and 20% methanol (v/v) flow rate 0.9mL/min.

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