Didodecyldimethyl ammoniumbromide

Didodecyldimethyl ammoniumbromide (DDAB) (Fluka, Bucks, Switzerland). Store at 4°C. 

The lipids (456 mg l-palmitoyl-2-oleyl-sn-glycero-3-phosphocholine, POPC and 5.6 mg didodecyldimethyl-ammoniumbromide, DDAB) in a 20-mL round bottom flask were dissolved in an appropriate amount of methylene chlo-ride/methanol (1 1, v/v, 20 mL). 

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