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Developing Orthogonal 2DLC Methods

DEVELOPMENT OF ORTHOGONAL 2DLC METHODS FOR SEPARATION OF PEPTIDES... [Pg.261]

Column Selection The selection of the two types of columns to be used is perhaps the most important consideration in 2DLC method development. This is driven by the need to have orthogonal dimensions for the solutes under investigation, otherwise the solutes will elute along the diagonal of the separation space, as discussed in Chapter 2. We have observed a number of 2DLC applications in the literature,... [Pg.132]

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