Determination of Sucrose by Invertase Hydrolase

The protein can be removed by the addition of 1N perchloric acid in the proportion of 1 1 as explained in Section 2.4.1. The solution should contain 0.4-0.8g glucose + sucrose per litre stronger solutions must be diluted. Remove excessive concentrations of glucose by treatment with glucose oxidase (Southgate 1991). 

Dilute 6.7 ml IM NaOH and 13.5 ml IM acetic acid with 180 ml of water. The pH should be 4.6. Bring buffer to 20-25 °C before use. (Buffer is stable for 1 year when stored at +4°C) 

Dissolve 10 mg of beta-fructosidase lyophilisate in 2 ml of distilled water. (Stable for 1 week when stored at +4°C) 

The following solution are the same as those used in Section 2.4.1. 

Bring buffer to 20-25 °C before use. (The buffer is stable for 4 weeks when stored at +4°C). 

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