Determination of distances at a supramolecular level using RET

Determination of distances at a supramolecular level using RET 2S3 where the amplitude-averaged decay times are defined as ... 

In addition to the determination of distances at a supramolecular level, RET can be used to demonstrate the mutual approach of a donor and an acceptor at a supramolecular level as a result of aggregation, association, conformational changes, etc. The donor and acceptor molecules are generally covalently linked to molecular, macromolecular or supramolecular species that move toward each other or move away. From the variations in transfer efficiency, information on the spatial relation between donor and acceptors can thus be obtained. Because of its simplicity, the steady-state RET-based method has been used in many diverse situations as shown below5 . 

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