Detergents, assay blocking solution

An ELISA-like sandwich assay is illustrated for a hypothetical array format in Fig. 1.1. Spots in the array are shown on an underlying nanoparticle bed, and may contain capture antibodies or aptamers on the spots to capture analyte proteins from the sample. After washing with detergent-protein solutions designed to block non-specific binding (NSB), a labeled secondary antibody is added to bind to captured analyte proteins. Enzyme labels catalyze conversion of an added chemical substrate to produce a colored product that is usually measured with an optical... 

---