Desolvation gas flow

Fig. 9.8 ESI MS plot after 90 min reaction and list of possible cyclopentyl silsesquioxanes with 1

Figure 8-50. Chromatographic conditions Column Sunfire C18,3.5 pm, 150 x 4.6 mm. Mobile phase lOmM NH4OAC, pH 5.8 acetonitrile, 25% acetonitrile to 75% acetonitrile over 10 min and 5-min hold at 75% acetonitrile. Wavelength 247 nm, column temperature, 35°C, flow, 1.3mL/min injection volume, lOpL MS conditions flow split 10 1. ESI -I- ion mode, single quadrupole, Z-Q. Capillary, + 3.5 kV cone, 25 V source temperature, 150°C desolvation temperature, 400°C cone gas flow, 113 L/hr desolvation gas flow, 419 L/hr.

The ESI source and tuning value parameters were as follows ionization mode API-ES positive, data acquisition full scan, mass range 120-1000 a.m.u., scan time 0.5 s, desolvation gas flow 615 Lh , cone gas flow 100 Lh, source temperature 115 °C, desolvation temperature 250 °C, cone voltage 32 V, capiUaiy voltage 2800 V. 

Fig. 3.38.The IUPAC names of Sudan azo dyes are as follows Sudan 1 = 1— [(2,4-dimethylphenyl)azo]-2-naphtalenol Sudan II = l-(phenylazo)-2-naphtol Sudan III = l-(4-phenylazophenylazo)-2-naphtol Sudan IV = o-tolyazo-o-tolyazo-beta-naphtol and Disperse Orange 13 = 4-[4-(phenylazo)-l-naphtylazo]-phenol. Azo dyes were separated in an ODS column (250 x 2.1 mm i.d. particle size 5 /xm) at 35°C. The isocratic mobile phase consisted of 0.1 per cent formic acid in methanol-0.1 per cent formic acid in water (97 3, v/v). The flow rate was 200 /xl/min. MS conditions were nebulizing and desolvation gas were nitrogen at the flow rates of 50 and 5551/h, respectively electrospray voltage, 3.0 kV cone voltage 25 V source temperature, 110°C desolvation temperature, 110°C. Azo dyes were extracted from the samples by homogenizing 1 g of sample with 10 ml of acetone, then the suspension was centrifuged and an aliquot of 3 ml of supernatant was mixed with 1 ml of deionized water, filtered and used for analysis. LC-ESI-MS/Ms SRM traces of standards and spiked samples are listed in Fig. 3.39. It was found that the detection and quantitation limits depended on both the chemical structure of the dye and the character of the accompanying matrix. LOD and LOQ values in chilli tomato sauce...

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