Deracemization of Primary Amines

The amoimt of pyruvate required for the deamination step could be drastically reduced by recycling the coproduct of the deamination step (d- or u-alanine) back to 

TABLE 2.6 Two-Step Deracemization of Pharmacologically Relevant Amines 

The economy of the process was significantly improved by immobilizing tiie first co-TAs since the catalyst can be easily removed by filtration after the first step and can be reused several times witiiout losing the activity [90]. (S)-Mexiletine was S5mthe-sized on a preparative scale in 95% 5deld and enantiopure form, demonstrating the practical applicability of die metiiodology. 

By combining the (S)-selective co-TA from Polaromonas sp. (PS-co-TA) and the (R)-selective TA from Neosarton/a fischeri (NY-co-TA) or Mycobacterium vanbaalenii (MW-co-TA), a panel of (R)-l-phenylethylamine derivatives (10 mM) was S5mthesized in high 5delds and enantiopure form (Table 2.7). 

Simultaneous deracemization of aromatic amines combining two stereocomplementary co-TAs using an enzyme-specific amine acceptor. 

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