Dependence of Enhanced Fluorescence Using SiFs

DISTANCE DEPENDENCE OF ENHANCED FLUORESCENCE USING SiFs 

The interactions of fluorophores with metallic particles have been the subject of a number of publications. There is reasonable agreement that the maximal enhancements occur within about lOOA from the surface, but some publications report the optimal distance to be as large as 600 A. In our opinion the use of metallic particles 

In summary, by the use of protein layers we found that the maximum increase in intensity and maximum decrease in lifetime were found for a single BSA-avidin layer which positions the fluorophore about 90 A from the surface. Hence the optimal distance for metakenhanced fluorescence (MEF) can be readily studied using protein monolayers. 

FITC-HSAIL=1) monoloyer plus 0.25/jM - Rhodomine B solution 

In conclusion, silver particles or colloids, when bound to a protein heavily labeled with fluorophores, can provide significantly higher intensities due to a decrease in the extent of self-quenching. 

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