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Deoxyribonucleic acid genetic engineering

The directed synthesis of deoxyribonucleic acid (DNA) and its manipulation for genetic engineering would be impossible without enzyme catalysis. There is a range of restriction enzymes which can break DNA strands at specific points defined by the local base sequence, as well as ligases which can rejoin the broken ends where new base sequences are inserted, and they are essential catalysts. They complement the chemical synthesis of the oligonucleotides (small segments of DNA) which are inserted. A discussion of the technique is outside the scope of this chapter, largely because the... [Pg.174]


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See also in sourсe #XX -- [ Pg.2 , Pg.157 ]

See also in sourсe #XX -- [ Pg.2 , Pg.157 ]




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