Deoxyhemoglobin cooperative effects

Substitutions in amino acids involved in subunit contacts can have several effects. In Hb Philly, a Tyr Phe change in the at a i j8, interface eliminates a hydrogen bond needed to stabilize deoxyhemoglobin (T-state) and leads to dissociation into monomers, which precipitate. This dissociation decreases cooperativity and increases oxygen... 

Cooper et al. in 1998 [35] performed another study on the adult brain. NIR was used to determine the effects of changes in the rate of oxygen delivery on adult rat-brain chemistry. Absolute levels of oxyhemoglobin, deoxyhemoglobin, and the redox state of the Cua center in mitochondrial cytochrome oxidase. A study on human infants was performed by Wyatt et al. [36]. They used NIR to quantify the cerebral blood volume in human infant. 

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