Densitometric plot

Quantitative Processing. Plates or film with the diffraction patterns were scanned with a Joyce-Loebl microdensitometer. Radial (20) densitometric plots of the crystalline pattern (eventually three successive exposures of the crystalline pattern are analyzed) and of the corresponding amorphous pattern were recorded on the same curve. In this way, the plot of the amorphous pattern was used as a reference standard. The densitometric recording began with the optical density of the non-irradiated emulsion this allowed the evaluation and normalization of the optical density of the diffraction pattern. When the analytical slit passed through the image of the border of the 75 pm objective aperture, the densitometric curve showed a sudden density raise "A d". (Fig. 6) The plots of the amorphous and crystalline patterns were thus normalized to the same reference " A d". Crystallinity was determined on the normalized curves by measuring the areas "C + A" and "A" under the crystalline and amorphous plots respectively. 

Figure 5 is an example of a densitometric plot for PETP,. The shoulder corresponding to the "amorphous halo" of the destroyed pattern is clearly visible. The normalization procedure is illustrated s in that specific case, the amorphous pattern is underexposed in comparison with the crystalline pattern. Point A corresponds to the optical density dQO of the background of the slide (that is outside the image of the border of the objective aperture) point B corresponds to the "initial" density dQ of the crystalline pattern and to the "initial" density d Q of the amorphous pattern if the crystalline plot is taken as the reference, the amorphous plot density has to be multiplied by the normalization factore i.e. 

Figures 8 and 9 are densitometric plots of various kinds of patterns. In figure 8, the procedure for the normalization of the amorphous plot is further explained. In this case, one has to multiply each individual density by the factor...

Figure 8. Densitometric plot of various kinds of patterns. The fiber is photographed in dark field conditions as opposed to other montages. (See also Figure 2.)...

Figure 9. Densitometric plot of various kinds of patterns...

Mix 4 pi of standards and samples with 4 pi of Soln. B. Spot 5 pi of each mixture onto a plastic foil (e.g., Parafilm), lying on a UV transilluminator. Photograph the spots and evaluate the picture densitometrically. The optical densities of the standards are plotted against the amount of their DNA and the DNA content of the samples is calculated from the obtained standard curve (range 0-15pgDNA/ml). 

In Fig. 9.5 is shown the first, and to our knowledge, only direct experimental plot of the volume of both the folded and unfolded states of a protein. The densitometric studies yielded directly the volume V of Snase as a function of temperature for the folded state (below the transition temperature, crosses) and for the unfolded state (above the transition temperature, diamonds). It can be seen as well from Fig. 9.5 that the increase in V of the native state of Snase with temperature is not linear indeed the folded state a decreases significantly as the temperature increases while at high temperature... 

Fig. 2. Exponential range for MDR-1 amplification in a renal cell carcinoma line. Top panel Ethidium-stained agarose gel demonstrating results of PCR amplification of MDR-1 mRNA in serially diluted cDNA. Bottom panel Densitometric values are plotted on the y-axis, while the dilution and the calculated amount of input RNA in the...

Fig. 4 Turnover of the 25 kDa apoprotein in wild-type and Chi b-less mutant strains of rice and barley. The peak area of the 25 kDa protein band determined from densitometric tracings of fluorogram were plotted against the chase time.

Densitometric measures enabled the determination of 2 /u,g of each enantiomer. A linear regression was observed plotting the sum of chromatographic area of the two enantiomers as a function of the amount of analyte applied to the layer. 

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