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Densitometric peak profiles

Terms of the Models Used to Simulate Densitometric Peak Profiles... [Pg.36]

Use of densitometric detection provides an insight into the concentration profiles of chromatographic bands, thus furnishing an indispensable prerequisite, needed for proper assessment of the retention mechanisms in the preparative adsorption TLC. Figure 2.4 shows three types of the band eoncentration profiles. The Gaussian peak (a) in this figure represents the linear isotherm of adsorption of a given species, peak... [Pg.20]

Figure 4-22. Densitometric analysis of serum separation. When electrophoresis is complete, the cellulose acetate film is removed and stained. Excess dye, not bound to protein, is removed by destaining and the film is transferred to a bath in which the cellulose acetate becomes transparent. The film is placed in a scanner in which it is moved across the light path of a spectrophotometer recording the absorbance of the blue stained protein peaks. The result is a densitometric scan whose peaks can be integrated to quantitate the protein profile of the serum. Figure 4-22. Densitometric analysis of serum separation. When electrophoresis is complete, the cellulose acetate film is removed and stained. Excess dye, not bound to protein, is removed by destaining and the film is transferred to a bath in which the cellulose acetate becomes transparent. The film is placed in a scanner in which it is moved across the light path of a spectrophotometer recording the absorbance of the blue stained protein peaks. The result is a densitometric scan whose peaks can be integrated to quantitate the protein profile of the serum.
The analogous, although considerably less pronounced changes in the respective values, the chromatographic peaks concentration profiles, and the specific rotation ([ckId) values were established by means of polarimetry and TLC with densitometric detection in the case of ibuprofen, naproxen, and 2-phenylpropionic acid, when stored as solutions in dichloromethane and physiological salt [2,3], Finally, we arrived at a conclusion that the true reason for all these oscillatory changes was a spontaneous and oscillatory transenantiomerization of the investigated APA solutions in the spirit of the classical Zhabotinskii-Belousov type oscillatory reactions. [Pg.236]


See other pages where Densitometric peak profiles is mentioned: [Pg.234]    [Pg.160]    [Pg.79]    [Pg.50]    [Pg.88]   
See also in sourсe #XX -- [ Pg.37 ]




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