Denaturation fully extended chain

Most denatured proteins are insoluble in water as a result of strong interactions between fully extended peptide chains. In the special case of S-sulfochymotrypsinogen and S-sulfotrypsinogen, aggregation may also occur. But, when special precautions are taken, water-clear solutions are obtained which are homogeneous in the ultracentrifuge (52). Under other circumstances (81), however, the S-sulfo derivatives appear to be poorly soluble in the pH range 3-9. Insolubility increases when reduction becomes more complete. 

---