Denaturation fast protein liquid chromatography

St. Martin, M. and Paquin, P. (1990). Ion exchange fast protein liquid chromatography, optimization of the purification of caseins using a non-denaturing detergent.. Dairy Res. 57, 63-68. 

Zhou et al. [5] found that at least seven components with fibrinolytic activity from earthworm E. fetida, Fig. (1). Three components are purified by fast protein liquid chromatography and proved to be homogenous on sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE), with apparent molecular masses 30,000, 25,000 and 30,000 Da, respectively. These enzymes are stable at pH 5 9, but totally denaturated below pH 2.6. 

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