Debranching enzyme direct

Branched cydodextrins are also used to increase the solubility of complexes. Two methods are used to make branched cydodextrins, an enzymic method and a pyrolytic method. In the enzymic method, a starch debranching enzyme, such as pul-lulanase, is added to a solution of cyclodextrin and a large excess of D-glucose or maltose to force the reaction to proceed in the reverse direction, i.e. to add rather than remove a branch.69 Since the equilibrium favors the debranching reaction, yields are low and the product typically contains —15% branched cyclodextrin and —85% glucose or maltose. Purification is difficult because of the high solubility of both the glucose or maltose and the branched cyclodextrin, but much of the unreacted cyclodextrin can be removed by crystallization. 

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