DEAE-celluIose

FIGURE 8. Fractionation of a fulvic acid sample on DEAE-celluIose using tris buffer plus a superimposed salt gradient as eluents. 

Previous work on human alkaline phosphatases has utilized chromatography (ElO) and starch-gel electrophoresis. Thus in 1956 Boman and Westlund (B34) reported the purification and separation of serum phosphatases by Dowex-2 column chromatography. Moss (M34) used gel filtration on Sephadex G-200 and DEAE-celluIose chromatography for separating 5 -nucleotidase and nonspecific alkaline phosphatase activities in human sera. In most of the studies of alkaline phosphatases in human tissues of liver (M33), intestine (M34, M35), bone (M36), kidney (B46), and urine (B44, B46, B47), crude extracts of these tissues were used and... 

DEAE-celluIose with CaS04 binder in HCI or in ammonia changes separation by binding purines in particular, whether as the base or the nucleoside. 

Fig. 2. Chromatographic diagram of porcine pancreatic juice (13, 14). Abbreviations used for enzyme names are the same as in Fig. 1. The anionic proteins are fractionated on a DEAE-cellulose column equilibrated with 0.005 M phosphate, pH 8.0 and eluted at the same pH with a concentration gradient. The cationic proteins are fractionated on a CM-celluIose column by stepwise elution with buffers of increasing pH s. Ordinates, optical density of the fractions at 280 m/j. Abscissas (on the right diagram), volume of eluate expressed in number of interstitial volumes of the column.

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