Daunorubicin poly

The dissociation kinetics of daunorubicin and daunorubicin actinomycin D from poly(dAdT).poly(dAdT) were studied using stopped-flow techniques. The data is summarized in Table IV. The dissociation of daunorubicin from poly(dAdT).poly(dAdT) is monophaslc with a lifetime of 0.2 sec. In the ternary complex (daunorubicin actinomycin D poly(dAdT).poly(dAdT)) the dissociation of the daunorubicin is characterized by two exponential decays, yielding lifetimes of 0.2 sec and 5 sec, A single 5 sec dissociation time was observed for actinomycin D. 

This data is consistent with a model in which daunorubicin is bound to Isolated DNA binding sites and dissociates with its normal lifetime of 0.2 sec. Whereas daunorubicin and actinomycin D molecules, residing at "adjacent sites", dissociate with a time constant of approximately 5 sec. These results are indicative of a cooperative interaction between daunorubicin and actinomycin D when the two are bound to poly(dAdT).poly(dAdT). 

Shen and Ryser developed daunorubicin-linked amino-ethyl polyacrylamide beads (Affi-gel 701) or poly(D-lysine) via an Af-cfr-aconityl spacer. The cis-aconityl linkage between drug and carrier was readily hydrolysed at pH 4 but not appreciably at pH 6. Poly(D-lysine) caused 90%... 

Zunino, F., GiuUiani, F., Savi, G., Dasdia, T. and Gambetta, R. (1982) Anti-tumour activity of daunorubicin linked to poly-L-aspartic acid. J. Pharmacol. Exp. Ther. 30 465-469. 

Tsukada Y, Kato Y, Umemoto N, Takeda Y, Kara T, Hirai H. An anti-oc-fetoprotein antibody-daunorubicin conjugate with a novel poly-L-glutamic acid derivative as intermediate drug carrier. J Natl Cancer Inst 73 1984 721-729. 

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