Daunomycin excretion

Rat liver canalicular membrane vesicles (CMV) have been used to examine the mechanisms of uptake of P-gp substrates such as daunomycin, daunorubicin, and vinblastine, whose biliary excretion is extensive (47,137, 408,409). Early work with plasma membrane vesicles, partially purified from MDR human KB carcinoma cells that accumulated [3H]vinblastine in an ATP-dependent manner, definitively showed how P-gp can act to efflux substrates from cancer cells (410). Additionally, these vesicles have been used to study microscopic aspects of P-gp-mediated efflux, such as the relationship of P-gp function to the membrane fluidity (137). 

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