Database hits, statistically significant

Hits on individual assays can be analyzed in a couple of different ways. First, drugs can be identified that have similar strength hits and then the ADR profiles of these drugs can be examined to identify ADRs that maybe associated with these hits. Also, contained in BioPrint are extensive collections of ADR associations [2], which have been identified by querying the database for statistically significant correlations between individual assays and individual ADRs. These ADRs are stored in the database and can be accessed by searching assay or the ADR. It is also useful to consult the pharmacokinetic data to confirm that the strength of the in vitro hit is consistent with in vivo exposure levels. 

Use the default parameters (except the number of alignments and descriptions) such as nr as the database (see Note 3), e-value 10 and the statistical significance threshold to include a sequence for generating the PSSM for the next iteration as 0.005. Change the maximum number of alignments and descriptions, 1000, from the respective pull down menus to retrieve possibly all statistically significant hits. 

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