Dansyl amino acid standards chromatogram

Figure 5. Chromatogram of dansyl amino acid standards. Column fused-silica capillary (0.32 mm i.d., 1.3 m length) packed with Aquapore RP-300 (10 jim) Mobile phase linear gradient from 5% to 32% 2-propanol in 0.05 M formic acid/0.06 M acetic acid buffer (pH 3.2) in 200 min, 2.0 pL/min, 55 C Sample injection 130 nL, 50-100 pmol per component.

Period 2 Part B—Work up the dansyl hydrolysate and spot on the TLC plate with standard dansyl amino acids. Part A. 1—Work up peptide hydrolysate and prepare FMOC derivatives of amino acids for analysis by HPLC or CE. Part A.2-If applicable, develop paper chromatogram in solvent system. 

Period 1 Part A—Begin acid hydrolysis of peptide. Part B—Complete dansyl chloride reaction and begin acid hydrolysis of dansyl peptide. Part A.2—if applicable, prepare paper chromatogram by applying standard amino acids. 

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